African Swine Fever Virus Indirect ELISA Antibody Detection Kit

Introduction

African Swine Fever (African Swine Fever) is a viral Infectious disease that is mandatory to report (List of Class A Diseases of the World Organization for Animal Health). Its outbreak will cause serious economic losses to pig farming countries. Therefore, rapid detection of infected pigs is essential to prevent the spread of the virus and control the spread of the disease. HWTAi® African Swine Fever Virus Indirect ELISA Antibody Detection Kit is based on the indirect ELISA method and is used to detect the African swine fever virus antibody in pig serum.

Benefits

1. One-step whole blood, serum and plasma testing.

2. Simple operation, non-professional on-site inspection.

3. The detection process is fast, and the result will be displayed in about 15 minutes.

4. The test time is less than or equal to 10 seconds, and it can detect 270 samples per hour at the fastest.

5. The detection equipment is compact, easy to carry, mobile terminal operation, cloud platform quality control.

6. The detection sensitivity is high, comparable to that of enzyme-labeled kits.

Principle

The recombinant ASF virus P30 antigen was pre-coated in the wells of the reaction plate. In the experiment, add the diluted serum to be tested, and after incubation, if the sample contains ASF virus-specific antibodies, it will bind to the antigen on the reaction plate; after washing, remove unbound antibodies and other components, and then Add enzyme conjugates to specifically bind to the antigen-antibody complex on the microtiter plate; then wash to remove unbound enzyme conjugates; add substrate solution to the microwell plate to form a blue product through enzyme catalysis, add After stopping the reaction with the stop solution, measure the absorbance OD value in the reaction well with a microplate reader at a wavelength of 450 nm.

Components

• Test devices

• Droppers

• Single Buffer

• IFU

Other Items

INTERPRETATION OF ASSAY RESULT