FCOV Ab Feline Coronavirus Antibody Rapid Test

Introduction

Feline Coronavirus (FCoV) is generally considered to be the cause of feline infectious peritonitis (FIP). The infection rate of the disease is very high, and it often occurs in clusters of cats, and is more likely to occur in young adult cats. The disease is divided into wet and dry types, and the clinical mortality rate is very high, reaching more than 90%. Clinically, infection with feline coronavirus does not necessarily cause the disease, but the detection of feline coronavirus can effectively prevent the spread of feline infectious peritonitis in cats. 

The FCOV Ab Feline Coronavirus antibody Rapid test is a test cassette to detect the presence of Feline Corona virus antibody (FCoV Ab) in cat's pleural fluid, ascitic fluid or serum specimen, to determine whetherare Fcov antibodies in the cat, it can be determined whether the cat is infected with the coronavirus or the vaccine has producted effective antibodies.

Benefits

1. One-step whole blood, serum and plasma testing.

2. Simple operation, non-professional on-site inspection.

3. The detection process is fast, and the result will be displayed in about 15 minutes.

4. The test time is less than or equal to 10 seconds, and it can detect 270 samples per hour at the fastest.

5. The detection equipment is compact, easy to carry, mobile terminal operation, cloud platform quality control.

6. The detection sensitivity is high, comparable to that of enzyme-labeled kits.

Components

• Test devices

• Droppers

• Single Buffer

• IFU

Principle

The FCOV Ab Feline Coronavirus antibody Rapid test is based on sandwich method lateral flow immunochromatographic assay. The test card has a testing window for the observation of assay running and result reading. The testing window has an invisible T (test) zone and a C (control) zone before running the assay. When the treated sample was applied into the sample hole on the device, the liquid will laterally flow through the surface of the test strip and react with the pre-coated FCoV recombinant antigens. If there are FCoV antibodies in the specimen, a visible T line will appear. The C line should always appear after a sample is applied, which indicates a valid result. By this means, the device can accurately indicate the presence of FCoV antibodies in the specimen.

Strage and stablity

The kit can be stored at room temperature (2-30°C). The test kit is stable through the expiration date (18 months) marked on the package label. DO NOT FREEZE. Do not store the test kit in direct sunlight.

Other Items

Test Procedure

       Collect cattle’s fresh whole blood, and get the serum or plasma specimen for use. Immediately apply the testing once collected the specimen.

• Take out the test device from the foil pouch and place it horizontally.

• Place 2-3 drop (around 70-100uL) of serum or plasma specimen into the sample hole. Start timer and wait for result.

• Interpret the result in 5-10 minutes. Result after 15 minutes is considered as invalid.

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INTERPRETATION OF ASSAY RESULT

- Positive (+): Whether the T line is clear or fuzzy, the "C" line and the region "T" line are present.

- Negative (-): Shows only the sharp C line. No T line.

- Invalid: no color lines in zone C. Whether the T line appears or not.