MycoplasmaTest kit

APPLICATION

For the isolation, identification, enumeration and antibiotic susceptibility tests of urogenital tract

Ureaplasma Urealyticum (UU) and Mycoplasma Hominis (MH) in one step.

TEST PRINCIPLE

The test kit consists of freeze-dried type culture broth and susceptibility test boards. The broth contains peptone, yeast extract, serum, growth factors and other nutrients, and urea, arginine as well. The alkaline material produced by urea, arginine decomposition causes the broth pH value rise, so the broth color changes from yellow to red. The broth has antibacterial agent which inhibits the growth of bacteria and fungi. The test board’s wells are used for control test and the UU and MH identification, enumeration and susceptibility tests.

MAIN COMPONENTS

1) Urea-arginine broth (freeze-dried): 20 vials

2) Susceptibility test board: 20 pcs

3) Sterile Pipette tip: 20 pcs

4) Sterile paraffin oil: 2 Bottles

5) Sterile diluting liquid: 2 Bottles

6) Instruction: 1 pcs

7) Report booklet: 1 pcs

STORAGE AND PERIOD OF VALIDITY

Stored at 2-8°C ; 12 months. SUSCEPTIBILITY FOR TEST

1) 10-antibiotic: Doxycycline (DOX), Minomyclin (MIN), Ciprofloxacin (CPF), Ofloxacin (OFL), Sparfloxacin (SPA), Roxithromycin (ROX), Azithromycin (AZI), Clarithromycin (CLA), Josamycin (JOS), Spectinomycin (SPE).

2) 12-antibiotic: Doxycycline (DOX), Minomyclin (MIN), Ciprofloxacin (CPF), Ofloxacin (OFL),

Sparfloxacin (SPA), Roxithromycin (ROX), Azithromycin (AZI), Clarithromycin (CLA), Josamycin

(JOS), Spectinomycin (SPE), Acetylspiramycin (ASP), Levofloxacin (LEV). 

SAMPLE REQUIREMENTS

Male: Urethral secretions, prostatic fluid, semen or urine sediment (Urine 10ml centrifugated at 2000 rpm for 10 minutes);

Female: Secretion from cervix opening or the amniotic fluid collected with Vaginal Speculum.

Or: 30-50μl freshly cultured positive broth.

RESULTS EXPLANATION

1) Identification and enumeration: Observe and keep the result of Ureaplasma Urealyticum (UU) after

24 hours; Observe and keep the result of Mycoplasma Hominis (MH) after 48 hours. The results are shown for example:

2) Antibiotic Susceptibility: Wells of line A contains antibiotics in lower concentration, while line B

higher. Wells A to B, one by one contains the same antibiotic. The results are shown for example:

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