FCOV Test Feline Corona Virus Antigen Test Kit

Introduction

FCOV Test Feline Corona Virus Antigen Test Kit is used to test FCOV, Feline Coronavirus (FCoV) is a common feline viral infection. Generally asymptomatic infection, but can cause mild diarrhea. Most cats infected with FCoV will clear the virus after infection, but some cats may develop a persistent infection. These cats are generally asymptomatic, can excrete large amounts of virus in their feces, and become a persistent source of infection for other cats in the environment. The continued circulation of FCoV in cats may increase the chance of FIP strains.

Benefits

1. One-step whole blood, serum and plasma testing.

2. Simple operation, non-professional on-site inspection.

3. The detection process is fast, and the result will be displayed in about 15 minutes.

4. The test time is less than or equal to 10 seconds, and it can detect 270 samples per hour at the fastest.

5. The detection equipment is compact, easy to carry, mobile terminal operation, cloud platform quality control.

6. The detection sensitivity is high, comparable to that of enzyme-labeled kits.

Components

• Test devices

• Droppers

• Single Buffer

• IFU

Principle

The Feline Panleukopenia/ Corona/ Giardia Antigen Combo Rapid test is based on sandwich lateral flow immunochromatographic assay.

Strage and stablity

The FCOV Test Feline Corona Virus Antigen Test Kit uses chromatographic immunoassay to qualitatively detect coronavirus antigens in cat feces. Load the sample to be tested on the sample pad, and then the capillary flows along the sample strip. The detection antibody is coupled to colloidal gold because its conjugate will be mixed with the sample solution. When FCoV antigen is present, a complex is formed by FCoV antigen and colloidal gold-labeled antibody. Then, the labeled antigen-antibody complex is bound by the second "capture-antibody" that recognizes the complex and fixed on the test strip as a T-line. Therefore, a positive result produces a visible burgundy line of antigen-antibody complexes. A burgundy C line will appear to confirm the correct operation of the test.

Other Items

Test Procedure

-Collect dog’s ocular, nasal or anus secretions with the cotton Swab and make the Swab wet sufficiently.
-Insert the Swab into the provided assay buffer tube. Agitates it to get efficient sample extraction. 
-If using serum or plasma specimen, use the dropper to collect the specimen. 

-Place 3 drops of serum or plasma into the assay buffer tube for dilution. The diluted sample is used as sample extraction. 
-Take out the test card from the foil pouch and place it horizontally.
-Suck the treated sample extraction from the assay buffer tube and place 3 drops into each sample hole “S” of the test card. 
-Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.

INTERPRETATION OF ASSAY RESULT

- Positive (+): Whether the T line is clear or fuzzy, the "C" line and the region "T" line are present.

- Negative (-): Shows only the sharp C line. No T line.

- Invalid: no color lines in zone C. Whether the T line appears or not.

Precaution

-All reagents must be tested at room temperature.

-Do not take the test tape out of the bag before use.

-Do not use the test after the expiration date.

-The components in this kit have been used as a standard batch processing unit for quality control testing. Do not mix parts of different batch numbers.

-All specimens are potentially infected. Must be treated strictly in accordance with the local country's rules and regulations.

Limitation 

Canivet cdv-cav-cvi-cpiv antigen combination test is only used for in vitro veterinary diagnosis. All results should be considered with other clinical data and can be discussed with the veterinarian. When a positive result is detected, it is recommended to use further confirmation methods such as RT-PCR.